Science always has been evolving and Man in his own realm has been inquisitive to reconnoiter the laws of nature and their insinuation on Human Life, especially. For this, Scientist’s life is eccentric around the laws of nature and to ponder over how these natural systems work. These enigmas always urge the scientist to be more rational and to delve about the esteem accomplishments of Nature. These conundrums were deciphered by the unfolding of genetic basis of life. And then, the norm of Genetic engineering was em-placed. This escorts the scientist to engender a synthetic organism by fabricating it artificially by employing the techniques and rules of Genetic engineering. The rationale to write this book is that it will help updating the contemporary knowledge in the field of Synthetic Biology and focuses more how the innovations are assembled by the backing of Genetic Engineering for the synthesis of Man Made Bacteria by applying different techniques and tools. This book will be the highlight because it is a broad-gauge of the history of achievements in the field of Man Made Biological Systems.