Freshney’s Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 8th edition

Freshney’s Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 8th edition

By R. Ian Freshney, Amanda Capes-Davis

Freshney’s Culture of Animal Cells is a comprehensive and informative resource that offers an in-depth understanding of the concepts surrounding basic methodology of cell culture. The eighth edition of this classic text has been fully updated to include the latest applications and techniques, such as iPSCs, CRISPR, and Organ-on-Chip technologies. The book aims to teach the readers both the how and why of tissue culture.

In this latest edition, the authors, R. Ian Freshney and Amanda Capes-Davis, have extensively revised the material to reflect the newest techniques and applications in the field. They cover a range of topics such as the use of CRISPR/Cas9 for gene editing, chemically defined conditions for stem cell culture, a brand-new chapter discussing the evolution of cell lines and the importance of reproducibility, and a dedicated chapter on the causes of and best practices for irreproducibility in research. Updated chapters and protocols cover topics including live-cell imaging, 3D culture, scale-up and automation, microfluidics, high-throughput screening, and toxicity testing.

The book is designed for undergraduate and graduate students, clinical and biopharmaceutical researchers, bioengineers, academic research scientists, and managers, technicians, and trainees working in cell biology, molecular biology, and genetics laboratories. Freshney’s Culture of Animal Cells is an indispensable volume for anyone involved in the field.

The authors have divided the book into four parts, with each part catering to a specific aspect related to animal cell culture. Part 1 delves into the biology of cultured cells and provides information on how to select media and substrates for cell growth. Part 2 discusses the practical aspects of tissue culture, including regulatory requirements, laboratory protocols, and aseptic technique. Part 3 discusses the methods for experimental manipulation of animal cells, covering topics such as transfection, cloning, hybridoma production, and cell synchronization. Part 4 concentrates on specialized techniques and applications in the field, such as organotypic cultures, microcarrier culture, and bioreactors.

Throughout the book, the authors have meticulously explained the concepts and techniques with the help of illustrations, diagrams, and tables, making it easier for the readers to understand the subject matter. The book also features a wealth of new content on iPSC generation and culture, new protocols for gene delivery, and the formation of tumor spheroids.

One of the most noteworthy features of the book is the companion website. The website includes supplementary protocols, figures, and artwork, which can be downloaded and printed to help the readers understand the concepts better. It is a valuable resource for students and researchers who are looking to delve into the subject matter more deeply.

Overall, Freshney’s Culture of Animal Cells is an essential and comprehensive guide for anyone who wishes to gain a thorough understanding of the techniques and applications of cell and tissue culture. The authors’ attention to detail and their expertise in the field make the book an excellent resource for students, researchers, and professionals alike.

If you are someone who is involved in the field of cell biology, molecular biology, or genetics, then this book is a must-have in your collection. The insights and understanding you will gain from this book will be invaluable to you in your career. You can order the paperback or digital kindle edition from various online stores such as Amazon, Barnes and Noble, or Waterstones. So go ahead and order your copy today, you wouldn’t want to miss out on this informative and comprehensive guide to animal cell culture!